Accelerated directed evolution of microbial consortia for the development of desirable plant phenotypic traits
AssigneeBiodiscovery New Zealand Limited
InventorsPeter Wigley, Caroline George, Susan Turner
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Abstract
The disclosure relates to methods for the screening, identification, and/or application of one or more microorganisms of use in imparting one or more beneficial properties to one or more plants.
Claims27
An iterative plant phenotypic trait based screening method for selecting one or more microorganisms capable of imparting at least one beneficial phenotypic trait to a plant, comprising:
- a) subjecting a plurality of plants to a growth medium in the presence of a first set of one or more microorganisms;
- b) growing the plurality of plants in said growth medium;
- c) selecting one or more plants from said plurality, following step b), based upon a beneficial plant phenotypic trait selection criteria, wherein said selected one or more plants exhibits the at least one or more beneficial phenotypic trait, as compared to other plants of said plurality;
- d) acquiring a second set of one or more microorganisms from said one or more plants, or from said one or more plants and the growth medium containing said one or more plants, selected in step c);
- e) repeating steps a) to d) one or more times, wherein the second set of one or more microorganisms acquired in step d) is used as the first set of microorganisms in step a) of any successive repeat;
- and
- f) selecting one or more microorganisms that is associated with imparting a beneficial phenotypic trait to a plant.
An iterative plant phenotypic trait based screening method of creating a microbial consortium capable of promoting at least one beneficial plant phenotypic trait, comprising:
- a) subjecting at least one plant to a growth medium, in the presence of a first plurality of microorganisms, and growing said plant in the growth medium;
- b) selecting at least one plant following step a) based upon a beneficial plant phenotypic trait selection criteria, wherein said selected plant exhibits the beneficial phenotypic trait, as compared to other plants of said plurality;
- c) acquiring a second plurality of microorganisms from said at least one plant selected in step b);
- d) repeating steps a) to c) one or more times, wherein the second plurality of microorganisms acquired in step c) is used as the first plurality of microorganisms in step a) of any successive repeat;
- e) isolating at least two microorganisms from said plurality of microorganisms that are associated with promoting at least one beneficial plant phenotypic trait;
- f) utilizing a molecular technique to characterize the at least two isolated microorganisms;
- g) selecting at least two microorganisms from the characterized microorganisms;
- and
- h) combining the at least two selected microorganisms into a microbial consortium.
An iterative plant phenotypic trait based screening method for selecting one or more microorganisms capable of imparting at least one beneficial phenotypic trait to a plant, comprising:
- a) subjecting a plurality of plants to a growth medium in the presence of a first set of one or more microorganisms;
- b) growing the plurality of plants in said growth medium;
- c) selecting one or more plants from said plurality, following step b), based upon a beneficial plant phenotypic trait selection criteria, wherein said selected one or more plants exhibits the at least one or more beneficial phenotypic trait, as compared to other plants of said plurality;
- d) acquiring a second set of one or more microorganisms from said one or more plants selected in step c);
- e) repeating steps a) to d) one or more times, wherein the second set of one or more microorganisms acquired in step d) is used as the first set of microorganisms in step a) of any successive repeat;
- and
- f) selecting one or more microorganisms that is associated with imparting a beneficial phenotypic trait to a plant.
Dependencies are inferred from the claim text.