Immune system modulating composition
AssigneeThe University Court of the University of Edinburgh
InventorJose Antonio Vazquez Boland
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Abstract
Rhodococcus equi (R.equi) has been determined to have a major adhesion factor encoded by a rpl pathogenicity island which enables host colonisation, wherein the rpl pathogenicity islandis absent from non-pathogenic Rhodococcus species. Further, the proteins (Rpl) encoded by the rpl pathogenicity islandhave been determined to be major immunodominant antigens. There is provided a novel diagnostic marker and vaccine candidate for R. equi in horses and other susceptible species.
Claims11
An isolated polypeptide associated with pilus formation in Rhodococcus equi comprising an amino acid sequence encoded by a polynucleotide sequence as set forth in SEQ ID NO:2, or an immunogenic fragment that has an amino acid sequence comprising DKITDLFDGFNFDDPGGE (SEQ ID NO:11) wherein the polypeptide or immunogenic fragment is linked to an immunogenic carrier.
A composition comprising a polypeptide associated with pilus formation in Rhodococcus equi comprising an amino acid sequence encoded by a polynucleotide sequence as set forth in SEQ ID NO:2, or an immunogenic fragment that has an amino acid sequence comprising DKITDLFDGFNFDDPGGE (SEQ ID NO:11), together with a pharmaceutically acceptable carrier.
A method of treating or preventing a disease or condition caused by Rhodococcus equi, comprising the step of administering an effective amount to a subject in need thereof of a polypeptide comprising an amino acid sequence encoded by a polynucleotide sequence as set forth in SEQ ID NO:2 or the immunogenic fragment that has an amino acid sequence comprising DKITDLFDGFNFDDPGGE (SEQ ID NO:11).
A method of detecting Rhodococcus equi wherein the method comprises detecting a polypeptide associated with Rpl pilus formation in Rhodococcus equi, comprising an amino acid sequence encoded by an isolated polynucleotide sequence as set forth in SEQ ID NO:2, or the immunogenic fragment that has an amino acid sequence comprising DKITDLFDGFNFDDPGGE (SEQ ID NO:11).
A composition capable of generating an immune response in a host comprising one or more surface-associated or secreted polypeptides of Rhodococcus equi wherein said polypeptides are isolated, and wherein said polypeptides are associated with formation of pili of Rhodococcus equi, wherein said composition comprises an isolated polypeptide comprising an amino acid sequence encoded by a polynucleotide sequence as set forth by SEQ ID NO:2 or the immunogenic fragment that has an amino acid sequence comprising DKITDLFDGFNFDDPGGE (SEQ ID NO:11) wherein the polypeptide or immunogenic fragment is linked to an immunogenic carrier, or a construct comprising an isolated nucleic acid molecule which encodes the isolated polypeptide wherein said isolated nucleic acid molecule is operably linked to a heterologous promoter which is functional to allow transcription of the nucleic acid sequence.
A method of inhibiting colonisation of a subject by Rhodococcus equi comprising the step of administering to a subject an effective amount of a polypeptide comprising an amino acid sequence encoded by a polynucleotide sequence as set forth in SEQ ID NO:
- 2 or the immunogenic fragmentthat has an amino acid sequence comprising DKITDLFDGFNFDDPGGE (SEQ ID NO 11).
Dependencies are inferred from the claim text.