Endotoxins having nematocidal activity and methods of use thereof
AssigneePioneer Hi-Bred International, Inc.
InventorsUte Schellenberger, Jun-Zhi Wei, Genhai Zhu
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Abstract
The invention provides novel δ-endotoxin polypeptides, and variants and fragments thereof, obtained from Bacillus thuringiensis having pesticidal activity against nematodes. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, biopesticide compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant parasitic nematodes.
Claims10
An expression cassette comprising a heterologous promoter operably linked to a polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising SEQ ID NO:
- 2;
- (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1;
- and
- (c) a nucleotide sequence encoding a polypeptide comprising residues 23-684 of SEQ ID NO:
- 1, wherein said amino acid sequence has nematicidal activity.
A plant comprising a heterologous polynucleotide operably linked to a promoter that drives expression in the plant, wherein said heterologous polynucleotide comprises a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising SEQ ID NO:
- 2;
- (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1;
- and
- (c) a nucleotide sequence encoding a polypeptide comprising residues 23-684 of SEQ ID NO:
- 1, wherein said amino acid sequence has nematicidal activity.
A microorganism comprising at least one heterologous polynucleotide operably linked to a promoter that drives expression in the microorganism, wherein said heterologous polynucleotide comprises a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising SEQ ID NO:
- 2;
- (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1;
- and
- (c) a nucleotide sequence encoding a polypeptide comprising residues 23-684 of SEQ ID NO:
- 1, wherein said amino acid sequence has nematicidal activity.
Dependencies are inferred from the claim text.