Method for screening a drug in retinal tissue
AssigneesResearch Foundation of the City University of New York, The Research Foundation of State University of New York
InventorsIoannis Danias, Hyungsik Lim
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Abstract
The subject matter disclosed herein pertains to a method for screening drugs using second-harmonic generation microscopy. The tissue is scanned with a pulsed laser light which has an excitation wavelength. At least some of microtubules within the tissue produce generated light with a second-harmonic wavelength that is half the excitation wavelength. A microtubule pattern within the tissue is determined based on an analysis of the generated second-harmonic wavelength.
Claims18
A method for screening drugs in a retinal tissue using label-free second-harmonic generation microscopy, the retinal tissue having a retinal nerve fiber layer comprising microtubules, the method comprising:
- exposing the retinal tissue to a drug;
- emitting a pulsed laser light having an excitation wavelength and a predetermined first polarization or a predetermined first spatial mode;
- scanning the retinal tissue with the pulsed laser light, at least some of the microtubules producing generated light with a second-harmonic wavelength that is half the excitation wavelength;
- measuring a generated polarization or a generated spatial mode of the generated light;
- determining a difference between (a) the first polarization and the generated polarization or (b) the first spatial mode and the generated spatial mode;
- and
- determining whether or not the drug impacts the retinal tissue based on the difference.
A method for screening drugs in a retinal tissue using label-free second-harmonic generation microscopy, the retinal tissue having a retinal nerve fiber layer comprising microtubules, the method comprising:
- exposing the retinal tissue to a drug;
- emitting a pulsed laser light having an excitation wavelength and a predetermined first spatial mode;
- scanning the retinal tissue with the pulsed laser light, at least some of the microtubules producing generated light with a second-harmonic wavelength that is half the excitation wavelength;
- measuring a generated spatial mode of the generated light;
- determining a difference between the first spatial mode and the generated spatial mode;
- and
- determining whether or not the drug impacts the retinal tissue based on the difference.
A method for determining a microtubule pattern in a retinal tissue using label-free second-harmonic generation microscopy, the retinal tissue having a retinal nerve fiber layer comprising microtubules, the method comprising:
- emitting a pulsed laser light having an excitation wavelength and a predetermined first polarization or a predetermined first spatial mode;
- scanning the retinal tissue with the pulsed laser light, at least some of the microtubules producing generated light with a second-harmonic wavelength that is half the excitation wavelength;
- measuring a generated polarization or a generated spatial mode of the generated light;
- determining a difference between (a) the first polarization and the generated polarization or (b) the first spatial mode and the generated spatial mode;
- and
- determining a microtubule pattern within the retinal tissue based on the second-harmonic wavelength.
Dependencies are inferred from the claim text.