Back to results
US 9,133,481 B2Granted
Semi-stable production of lentiviral vectors
AssigneeMolMed SpA
InventorsChiara Bovolenta, Anna Stornaiuolo, Paolo Rizzardi, Fulvio Mavilio
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeMolMed SpA
InventorsChiara Bovolenta, Anna Stornaiuolo, Paolo Rizzardi, Fulvio Mavilio
Estimating the term…
The present invention provides a new semi-stable packaging cell line and a method to produce lentiviral vectors (LV), using the semi-stable packaging cell line. New methods and packaging cell lines of the invention are generated using a baculo-AAV hybrid system for stable expression of structural and regulatory lentiviral proteins, such system comprising a baculoviral backbone containing an integration cassette flanked by AAV ITRs, in combination with a plasmid encoding rep protein. This system allows to obtain a stable integration of the structural and regulatory HIV-1 proteins gag/pol and rev. The system allows to obtain a cell line including the structural and regulatory HIV proteins gag/pol and rev, to be used for a semi-stable LV production.
A system for stable expression of lentiviral structural and regulatory proteins consisting of:
A semi-stable lentiviral packaging cell line consisting of cells stably expressing lentiviral gag, pol and rev characterized in that such cells contain stably integrated into its genome at least one copy of an integration cassette flanked by AAV inverted terminal repeats (ITR) including two expression cassettes, wherein the first expression cassette encodes lentiviral gag and pol and the second cassette encodes lentiviral rev and a selection marker.
Dependencies are inferred from the claim text.