Systems, methods and compositions for optimizing tissue and cell enriched grafts

An adipose tissue processing device, comprising:

• a flexible, collapsible bag comprising a first outer shell and a second outer shell that are joined to form a bag, wherein the bag comprises:
• a first port in communication with a first subsystem, which receives adipose tissue and, which is defined by a first outer shell of the bag and a filter disposed within the bag, wherein the filter comprises a plurality of pores that pass liquids, tumescent fluids, red blood cells, and wash solutions and retain mature adipocytes, regenerative cells, stem cells, progenitor cells and connective tissue;
• and
• a second port in communication with a second subsystem, which is defined by the filter and the second outer shell of the bag,
• wherein the second subsystem further comprises a separator that creates a space between the filter and the second outer shell of the bag,
• wherein said separator comprises a separation screen or mesh that comprises pores that are larger than the pores in the filter,
• wherein the first subsystem and second subsystem are configured to store tissue or cell samples within the bag as the flexible, collapsible bag expands, wherein the pore size of said filter is between about 30 μm and about 200 μm, and
• wherein the separator comprises pores that comprise a pore size that is between about 300 and 2000 μm.