High throughput multiwell system for culturing 3D tissue constructs in-vitro or in-vivo, method for producing said multiwell system and methods for preparing 3D tissue constructs from cells using said multiwell system

A method for producing a multiwell system for use for preparing a 3D tissue construct from cells, comprising steps of:

• a) melting alginate, polylactic acid, or PEOT/PBT in a thermal jacket;
• b) extruding the melted alginate, polylactic acid, or PEOT/PBT under pressure through a nozzle to form a plotted fiber;
• c) repeating step b), thereby depositing a subsequent plotted fiber in parallel at a distance of between 0.125 and 2.0 mm next to the last deposited fiber until a layer of alginate, polylactic acid, or PEOT/PBT is formed;
• d) depositing a subsequent layer on the previous layer, wherein the fibers forming said subsequent layer are deposited at an angle preferably between 10 and 90 degrees relative to the fibers of the previous layer;
• and
• e) repeating step d) until a desired height is achieved;
• wherein the method produces a multiwell system comprising at least 3 wells, the wells comprise sidewalls made of alginate, polylactic acid or PEOT/PBT, wherein said wells have a volume between 0.125 and 4.0 mm3, a wall thickness of at least 50 microns and a closed bottom, and wherein the multiwell system does not comprise a 3D synthetic polymer scaffold.