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US 9,133,406 B2Granted
Production of fatty acid derivatives
AssigneeReg Life Sciences, LLC
InventorAlfred Gaertner
Life of this patent
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Abstract
Methods and compositions for producing fatty acid derivatives, for example, fatty esters, and commercial fuel compositions comprising fatty acid derivatives are described.
Claims12
A method of producing a fatty acid ester, the method comprising:
- culturing a recombinant bacterium genetically engineered to express an exogenous ester synthase (EC 2.3.1.75) having at least 90% sequence identity to SEQ ID NO:
- 18 and having an amino acid substitution at the residue corresponding to glycine 395 of SEQ ID NO:
- 18 in culture medium comprising a carbon source, under conditions effective to express the ester synthase, wherein said ester synthase has an increased enzymatic activity relative to that of SEQ ID NO:
- 18.
1 more element
Dependencies are inferred from the claim text.