Hatching fluid enzymes and uses thereof
AssigneeAqua Bio Technology Asa
InventorsHans Kristian Leren, Bernt Th. Walther
Life of this patent
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Abstract
The present invention relates to various polypeptides from fish hatching fluid, their encoding nucleic acid sequences, pharmaceutical compositions comprising said polypeptides and nucleic acid molecules and their use in various medical and cosmetic applications to the skin, particularly for moisturizing skin and/or for exfoliation of the horny layer of the skin for treating or preventing skin disorders or conditions in an animal.
Claims17
A pharmaceutical or cosmetic composition comprising:
- (i) an isolated polypeptide having metalloproteinase activity comprising an amino acid sequence as set forth in SEQ ID NO:1;
- or
- (ii) an isolated polypeptide having metalloproteinase activity comprising an amino acid sequence which is at least 90% identical to the sequence as set forth in SEQ ID NO:1;
- or
- (iii) an isolated polypeptide having metalloproteinase activity comprising a portion of the amino acid sequence as set forth in SEQ ID NO:1, wherein said portion comprises at least 150 amino acids;
- or
- (iv) an isolated polypeptide having metalloproteinase activity comprising a portion of an amino acid sequence which is at least 90% identical to a comparable region of the amino acid sequence as set forth in SEQ ID NO:1 and comprises at least 150 amino acids;
- and/or
- (v) one or more isolated nucleic acid molecules encoding a polypeptide as set forth in any of (i) to (iv) above or a sequence fully complementary thereof,
- an effective amount of an added stabilizing agent wherein the stabilizing agent stabilizes the isolated polypeptide having metalloproteinase activity or the isolated one or more nucleic acid molecules against degradation, and
- one or more pharmaceutically or cosmetically acceptable excipients and/or diluents, wherein the composition is a gel, cream, ointment, lotion, foam, non-aqueous solution, spray, salve, stick, soap, powder, film, emulsion, suspension or dispersion.
A cosmetic or non-cosmetic method of exfoliating and/or moisturizing skin of an animal, wherein an effective amount of a cosmetic or a pharmaceutical composition is topically administered to said animal, wherein said cosmetic or pharmaceutical composition comprises:
- (i) an isolated polypeptide having metalloproteinase activity comprising an amino acid sequence as set forth in SEQ ID NO:1;
- or
- (ii) an isolated polypeptide having metalloproteinase activity comprising an amino acid sequence which is at least 90% identical to a sequence as set forth in SEQ ID NO:
- 1;
- or
- (iii) an isolated polypeptide having metalloproteinase activity comprising a portion of an amino acid sequence as set forth in SEQ ID NO:
- 1, wherein said portion comprises at least 150 amino acids;
- or
- (iv) an isolated polypeptide having metalloproteinase activity comprising a portion of an amino acid sequence which is at least 90% identical to a comparable region of an amino acid sequence as set forth in SEQ ID NO:
- 1 and comprises at least 150 amino acids;
- and/or
- (v) one or more isolated nucleic acid molecules encoding a polypeptide as set forth in any of (i) to (iv) above or a sequence fully complementary thereof,
- and one or more pharmaceutically or cosmetically acceptable excipients and/or diluents.
Dependencies are inferred from the claim text.