Modified polypeptide having homoserine acetyltransferase activity and microorganism expressing the same
AssigneeCj Cheiljedang Corporation
InventorsSo Young Kim, Yong Uk Shin, Chang Il Seo, In Kyung Heo, Ju Eun Kim, Hyun Ah Kim, Han Jin Lee, Kwang Ho Na, Sung Kwang Son
Life of this patent
Estimating the term…
Abstract
The present invention relates to a polypeptide that is modified to have homoserine O-acetyltransferase activity, and in particular, the present invention provides a modified polypeptide having homoserine O-acetyltransferase activity, in which the amino acid at position 111 of a polypeptide having homoserine succinyltransferase activity is substituted with other amino acid.
Claims23
A polypeptide having homoserine O-acetyltransferase activity that is at least 95% homologous to the amino acid sequence of SEQ ID NO:
- 17, wherein the amino acid at position 111 from the start amino acid methionine of the sequence is substituted with glutamic acid, and the amino acid at position 112 of the sequence is substituted with threonine or histidine.
A polypeptide having homoserine O-acetyltransferase activity that is at least 95% homologous to the amino acid sequence of SEQ ID NO:
- 17, wherein
- the amino acid at position 111 from the start amino acid methionine of the sequence is substituted with glutamic acid,
- the amino acid sequence is further substituted with proline at position 29, substituted with glycine at position 114, substituted with serine at position 140, or one or more combinations of them, and
- the amino acid sequence is further substituted with threonine or histidine at position 112.
A polypeptide having homoserine O-acetyltransferase activity that is at least 95% homologous to the amino acid sequence of SEQ ID NO:
- 17, wherein
- the amino acid at position 111 from the start amino acid methionine of the sequence is substituted with glutamic acid,
- the amino acid sequence is further substituted with proline at position 29, substituted with glycine at position 114, substituted with serine at position 140, or one or more combinations of them, and
- the polypeptide exhibits resistance to feedback regulation by methionine, and has the amino acid sequence of SEQ ID NO:
- 21.
Dependencies are inferred from the claim text.