Method for production of reprogrammed cell using chromosomally unintegrated virus vector
AssigneeDNAVEC Corporation
InventorsNoemi Fusaki, Hiroshi Ban, Mamoru Hasegawa, Yoshikazu Yonemitsu
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Abstract
An objective of the present invention is to provide vectors for conveniently and efficiently producing ES-like cells in which foreign genes are not integrated into the chromosome. The present inventors discovered methods for producing ES-like cells from somatic cells using chromosomally non-integrating viral vectors. Since no foreign gene is integrated into the chromosome of the produced ES-like cells, they are advantageous in tests and research, and immunological rejection and ethical problems can be avoided in disease treatments.
Claims4
A method of producing an induced pluripotent stem cell (iPSC), comprising introducing one or more Sendai virus vectors encoding at least Oct3/4 and Sox2 into a differentiated cell, and culturing said cell under conditions to obtain a reprogrammed iPSC.
Dependencies are inferred from the claim text.