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US 9,121,044 B2Granted
Biosynthesis of caffeic acid and caffeic acid derivatives by recombinant microorganisms
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsYajun Yan, Yuheng Lin
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Abstract
Microorganisms are genetically engineered to synthesize caffeic acid from simple carbon sources via a tyrosine intermediate by means of a dual pathway that utilizes both endogenous and engineered enzymatic activities.
Claims16
A method for producing caffeic acid comprising:
- culturing a genetically engineered cell comprising p-coumarate 3-hydroxylase (C3H) enzyme activity, wherein said cell has been metabolically engineered to express or overexpress a tyrosine ammonia lyase (TAL) and to overexpress a 4-hydoxyphenylacetate 3-hydroxylase (4HPA3H) enzyme or enzyme complex, under conditions effective to produce caffeic acid;
- and
- isolating the caffeic acid from the cell or the culture medium.
Dependencies are inferred from the claim text.