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US 9,120,849 B2Granted
Nucleic acid amplification controls
AssigneeZeptoMetrix Corporation
InventorsGregory R. Chiklis, James C. D. Hengst
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Abstract
The present invention discloses positive control material for nucleic acid amplification based detection of microorganisms in biological samples. The control material comprises purified microorganism that is rendered non-infectious but is amenable to nucleic acid amplification. Also disclosed is a process for making and using the control material.
Claims9
A method for producing a positive control for the detection of microorganisms which comprises
- (a) purifying microorganisms from a source;
- (b) exposing the purified microorganism to an aldehyde at a temperature of 2-8° C. such that one or more surface proteins are irreversibly modified while leaving the nuclear components substantially intact, and the purified microorganism is thereby rendered non-pathogenic and is capable of nucleic acid amplification;
- and (c) suspending the microorganism in a liquid matrix comprising a biological fluid or a synthetic biological fluid,
- wherein the composition is useful as a positive internal control for nucleic acid amplification detection tests.
1 more element
Dependencies are inferred from the claim text.