Loading patent…
AssigneeKorea Advanced Institute of Science and Technology
InventorsSang Yup Lee, Zhi Gang Qian, Xiaoxia Xia, Min Young Kim
Estimating the term…
The present invention relates to a mutant microorganism having a high ability to produce cadaverine, and a method for producing cadaverine using the same. More specifically, the invention relates to a mutant microorganism having a high ability to produce cadaverine wherein a gene involved in the cadaverine degradation or utilization pathway is inactivated or deleted, and to a method for producing cadaverine in high yield by culturing the mutant microorganism under aerobic conditions. The mutant microorganism according to the present invention is useful for producing a high yield of cadaverine which can be widely used in various industrial applications.
A mutant microorganism having the ability to produce cadaverine wherein the speE gene encoding putrescine/cadaverine aminopropyl transferase, the speG gene encoding spermidine N-acetyltransferase, the ygjG gene encoding putrescine/cadaverine aminotransferase, the puuP gene encoding putrescine importer, and the puuA gene encoding glutamate-putrescine/glutamate-cadaverine ligase, which are involved in the cadaverine degradation or utilization pathway, are inactivated or deleted, wherein the cadA gene encoding lysine decarboxylase is further amplified or introduced into said mutant microorganism, and wherein the native promoter of at least one gene selected from the group consisting of a dapA gene encoding dihydrodipicolinate synthase, a lysA gene encoding diaminopimelate decarboxylase, and a dapB gene encoding dihydrodipicolinate reductase are replaced with a strong promoter.
A method for preparing a mutant microorganism having the ability to produce cadaverine, the method comprising:
Dependencies are inferred from the claim text.