Vault and vault like carrier molecules

A method of delivering at least one substance to an organism, comprising administering to the organism an assembled vault-like particle, wherein the assembled vault-like particle comprises:

• i) multiple copies of a naturally occurring sequence of MVP or a naturally occurring sequence of MVP having an N-terminal and a C-terminal and having at least one modification selected from the group consisting of addition of an amino acid sequence to the C-terminal, addition of an amino acid sequence to the N-terminal, deletion of between 1 to 100 amino acid residues from the C-terminal, deletion of between 1 to 100 amino acid residues from the N-terminal, and substitution of at least one amino acid residue of the naturally occurring sequence of MVP,
• ii) a naturally occurring sequence of VPARP, a portion of the naturally occurring sequence of VPARP comprising at least about 150 consecutive residues from residue 1473 to residue 1724 of VPARP SEQ ID NO:
• 3, or an intraspecies variation in the sequence thereof in the assembled vault-like particle, wherein the naturally occurring sequence of VPARP or the portion of the naturally occurring sequence of VPARP has at least one modification selected from the group consisting of addition of an amino acid sequence to the C-terminal of the naturally occurring sequence of VPARP or the portion of the naturally occurring sequence of VPARP and addition of an amino acid sequence to the N-terminal of the naturally occurring sequence of VPARP or the portion of the naturally occurring sequence of VPARP, and
• iii) the at least one substance.