Food additive comprising an amidase for detoxifying ochratoxin
AssigneeDupont Nutrition Biosciences Aps
InventorsShukun Yu, Charlotte Horsmans Poulsen, Soren Dalsgaard, Huaming Wang, Igor Nikolaev
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Abstract
The present invention relates to amidase enzymes and a feed or food additive comprising the amidase enzyme capable of degrading ochratoxin.
Claims29
An isolated amidase enzyme capable of degrading ochratoxin, wherein the isolated amidase enzyme comprises a polypeptide sequence having a sequence of SEQ ID NO:
- 3 that has a cleavable tag, or a sequence having at least 70% sequence identity to SEQ ID NO:
- 3 that has the cleavable tag.
A feed or food composition comprising an amidase enzyme capable of degrading ochratoxin, wherein the amidase enzyme comprises a polypeptide sequence having a sequence of SEQ ID NO:
- 3 or a polypeptide sequence having at least 70% sequence identity to SEQ ID NO:
- 3, wherein said feed or food composition is selected from the group consisting of:
- grains, by products from cereals, silage, protein, oils and fats, and minerals and vitamins.
A method of reducing ochratoxin contamination of a feed or food composition comprising adding to said feed or food composition an ochratoxin degrading amidase enzyme, wherein the ochratoxin degrading amidase enzyme comprises a polypeptide sequence having a sequence of SEQ ID NO:
- 3 or a polypeptide sequence having at least 70% sequence identity to SEQ ID NO:
- 3, wherein said feed or food composition is selected from a group consisting of:
- grains, by products from cereals, silage, protein, oils and fats, and minerals and vitamins.
A recombinant cell or spore encoding an amidase enzyme which degrades ochratoxin, wherein the amidase enzyme comprises a polypeptide sequence having a sequence of SEQ ID NO:
- 3 that has a cleavable tag, or a sequence having at least 70% sequence identity to SEQ ID NO:
- 3 that has the cleavable tag.
Dependencies are inferred from the claim text.