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US 9,103,842 B2Granted
Methods for rejuvenating red blood cells
AssigneeBiomet Biologics, LLC
InventorsAlan Gray, Joel C. Higgins, Matthew D. Landrigan, Grant Cunningham
Life of this patent
Estimating the term…
Abstract
Methods for transfusing blood to a mammalian subject. The method includes obtaining a unit of blood, rejuvenating the blood with an enhancement composition, measuring 2,3-DPG concentration of the blood, and delivering the blood to a subject.
Claims20
A method for treating or preventing ischemia in a human or other mammalian subject, comprising:
- (a) obtaining a volume of fresh blood from the subject;
- (b) performing a separation on the fresh blood, wherein plasma and leukocytes are delivered to the subject, and fresh red blood cells are isolated;
- (c) rejuvenating the fresh red blood cells with an enhancement composition to form rejuvenated fresh red blood cells, wherein the rejuvenated fresh red blood cells have adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG) levels equal to or greater than ATP and 2,3-DPG levels normally found in the subject's blood;
- and
- (d) delivering the rejuvenated fresh red blood cells to the subject, wherein the performing a separation, rejuvenating and delivering are performed within about 24 hours of the time of obtaining the fresh blood from the subject.
2 more elements
A method of enhancing the ability of blood to oxygenate tissue in a subject in need thereof comprising:
- (a) obtaining a unit of fresh blood;
- (b) performing a first diagnostic test on the fresh blood to determine the concentration of a marker selected from 2,3-diphosphoglycerate (2,3-DPG), adenine triphosphate (ATP), or combinations thereof;
- (c) contacting the fresh blood with an enhancement composition to form rejuvenated fresh blood;
- (d) performing a second diagnostic test on the rejuvenated fresh blood to determine the concentration of the marker;
- (e) comparing the concentration of the marker from the first diagnostic test to the concentration of the marker from the second diagnostic test and determining a volume of rejuvenated fresh blood necessary to dose the subject to normal or higher levels of 2,3-DPG and/or ATP in the subject's systemic circulation;
- and
- (f) administering the volume of rejuvenated fresh blood, determined in step (e) to the subject;
- wherein the obtaining, performing a first diagnostic test, contacting, performing a second diagnostic test, comparing and administering are performed within about 24 hours of the time of obtaining the fresh blood from the subject.
5 more elements
A method of performing a transfusion comprising:
- (a) obtaining a unit of fresh blood;
- (b) performing a diagnostic test on the unit of fresh blood to determine 2,3-diphosphoglycerate (2,3-DPG) concentration in the blood;
- (c) rejuvenating the fresh blood if the 2,3-DPG concentration is lower than about 10 μmol/L/gHb to form rejuvenated fresh blood, wherein the rejuvenating is performed with an enhancement composition comprising, inosine, adenine, pyruvate, and sodium phosphate;
- and
- (d) delivering the rejuvenated fresh blood to a subject in need thereof;
- wherein the obtaining, performing a diagnostic test, rejuvenating and delivering are performed within about 24 hours of the time of obtaining the fresh blood from the subject.
3 more elements
A method for treating or preventing ischemia in a human or other mammalian subject, comprising:
- (a) obtaining a volume of fresh blood from the subject;
- (b) performing a separation on the fresh blood, wherein plasma and leukocytes are delivered to the subject, and fresh red blood cells are isolated;
- (c) rejuvenating the fresh red blood cells with an enhancement composition to form rejuvenated fresh red blood cells, wherein the concentration of 2,3-diphosphoglycerate (2,3-DPG) concentration in the rejuvenated red blood cells is greater than about 15 μmol/L/gHb;
- and
- (d) delivering the rejuvenated fresh red blood cells to the subject;
- wherein the obtaining, performing a separation, rejuvenating, and administering are performed within about 24 hours of the time of obtaining the fresh blood from the subject.
3 more elements
Dependencies are inferred from the claim text.