Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage
AssigneeInvista North America S.A.R.L.
InventorsAdriana Leonora Botes, Alex Van Eck Conradie
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Abstract
This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogs enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.
Claims26
A method for biosynthesizing one or more products selected from the group consisting of adipic acid, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine, caprolactam, and 1,6-hexanediol, said method comprising enzymatically synthesizing hexanoyl-CoA and enzymatically forming, in one or more steps, two terminal functional groups selected from the group consisting of carboxyl, amine, and hydroxyl groups in hexanoyl-CoA to produce the one or more products.
Dependencies are inferred from the claim text.