Method for producing virus vector
AssigneeTakara Bio Inc.
InventorsKazuhisa Shinmura, Yoshinori Katayama, Kensuke Sakai, Toshihiro Shodai, Hirofumi Yoshioka, Junichi Mineno
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Abstract
The present invention provides a method for producing a virus vector, which comprises a step wherein cells that are capable of producing a virus vector are cultured in a culture medium that contains, as active components, a retinoic acid and a histone deacetylase inhibiting substance; and a culture medium for the production of a virus vector, which is characterized by containing, as active components, a retinoic acid and a histone deacetylase inhibiting substance.
Claims7
A method of producing a retrovirus particle, which comprises a step of culturing a producer cell line which produces the retrovirus particle and producing the retrovirus particle in a culture medium containing retinoic acid and a histone deacetylase inhibitor as active ingredients and a step of harvesting a supernatant containing the retrovirus particle from the culture medium,
- wherein the culture medium contains either 100 nM to 3 μM trichostatin A or 1 mM to 10 mM sodium butyrate as the histone deacetylase inhibitor, and
- wherein the method provides retrovirus particles having higher gene transduction efficiency as compared to culture conditions not comprising retinoic acid and a histone deacetylase inhibitor.
Dependencies are inferred from the claim text.