Compositions and methods of using chondroitinase ABCI mutants
AssigneeAcorda Therapeutics, Inc.
InventorsAnthony O. Caggiano, Andrea Vecchione, Jennifer Iaci
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Abstract
The present disclosure relates to protein and nucleic acid mutants of chondroitinase ABCI. Such nucleic acid mutants encode for chondroitinase ABCI mutant enzymes exhibiting altered chondroitin lyase activity or increased resistance to inactivation from stressors including UV light or heat. Methods of using such nucleic acid mutants encoding chondroitinase ABCI mutant enzymes is also provided.
Claims21
An isolated nucleic acid sequence comprising a nucleotide sequence selected from SEQ ID NO:
- 10, SEQ ID NO:
- 13, and SEQ ID NO:
- 14, encoding a mutant chondroitinase ABCI enzyme.
An expression vector comprising an isolated nucleic acid sequence comprising a nucleotide sequence selected from SEQ ID NO:
- 10, SEQ ID NO:
- 13, and SEQ ID NO:
- 14, encoding a mutant chondroitinase ABCI enzyme.
A method of making an expression vector comprising:
- ligating an isolated nucleic acid sequence comprising a nucleotide sequence selected from SEQ ID NO:
- 10, SEQ ID NO:
- 13, and SEQ ID NO:
- 14, encoding a mutant chondroitinase ABCI enzyme, to a vector nucleic acid in the expression vector.
Dependencies are inferred from the claim text.