Trehalose phosphorylases and their use in the biocatalytic production of trehalose-analogues and glycosyl phosphates
AssigneeUniversiteit Gent
InventorsJef Van Der Borght, Tom Desmet, Wim Soetaert, Chao Chen
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Abstract
The present invention relates to trehalose phosphorylases which are useful for the industrial production of trehalose-analogs and glycosyl phosphates. More specifically, the invention discloses trehalose phosphorylases which are mutated in specific amino acid regions. These specific mutations result in modified substrate specificities of the enzymes. In addition, the present invention discloses a wild type trehalose phosphorylase from the marine organism Caldanaerobacter subterraneus, and mutated types thereof, which are highly thermostable and have a broad acceptor and donor specificity.
Claims9
A mutated trehalose phosphorylase comprising the amino acid sequence of SEQ ID NO:
- 1, except for a mutation in one or more of amino acid positions 371, 442, 450, 649, 659, or 693, and wherein said mutated trehalose phosphorylase has an increased catalytic efficiency towards its acceptor or donor substrate compared to the corresponding wild-type trehalose phosphorylase.
Dependencies are inferred from the claim text.