Immobilised biological entities
AssigneeW.L. Gore & Associates, Inc.
InventorsKarin Leontein, Per Antoni, Daniel Nyström, Paul Begovac, Krzysztof Pietrzak
Life of this patent
Estimating the term…
Abstract
There is described inter alia a device having a surface comprising a layered coating wherein the outer coating layer comprises a plurality of cationic hyperbranched polymer molecules characterized by having (i) a core moiety of molecular weight 14-1,000 Da (ii) a total molecular weight of 1,500 to 1,000,000 Da (iii) a ratio of total molecular weight to core moiety molecular weight of at least 80:1 and (iv) functional end groups, whereby one or more of said functional end groups have an anti-coagulant entity covalently attached thereto.
Claims30
A device having a surface comprising a layered coating wherein the outer coating layer comprises a plurality of cationic hyperbranched polymer molecules characterized by having (i) a core moiety of molecular weight 14-1,000 Da (ii) a total molecular weight of 10,000 to 300,000 Da (iii) a ratio of total molecular weight to core moiety molecular weight of at least 80:1 and (iv) functional end groups, whereby one or more of said functional end groups have a heparin moiety covalently attached thereto, wherein said heparin moiety is single point covalently attached to the hyperbranched polymer molecule, and wherein the hyperbranched polymer is not a dendrimer.
A non-thrombogenic device which is obtainable by a process comprising:
- (a) treating a device to present a surface coating comprising an outer coating layer comprising cationic hyperbranched polymer molecules characterized by having (i) a core moiety of molecular weight 14-1,000 Da (ii) a total molecular weight of 10,000 to 300,000 Da and (iii) a ratio of total molecular weight to core moiety molecular weight of at least 80:1 (e.g. at least 100:1) and bearing functional end groups, wherein the hyperbranched polymer is not a dendrimer;
- (b) reacting at least one or more of said functional end groups with molecules of a heparin moiety which is functionalized to bear groups which are capable of reacting with the reactive functional groups on the hyperbranched cationic polymer;
- thereby to attach the heparin moiety to the hyperbranched cationic polymer, wherein the heparin moiety is single point covalently attached to the hyperbranched polymer.
A non-thrombogenic device which is obtainable by a process comprising:
- (a) treating a device to present a positively charged polymer surface layer;
- and
- (b) associating with said polymer surface layer functionalized cationic hyperbranched polymer molecules characterized by having (i) a core moiety of molecular weight 14-1,000 Da (ii) a total molecular weight of 10,000 to 300,000 Da and (iii) a ratio of total molecular weight to core moiety molecular weight of at least 80:1 (e.g. at least 100:1) and bearing a multiplicity of negatively charged heparin moieties and wherein said functionalized hyperbranched cationic polymer has a net negative charge and is not a dendrimer;
- or
- (a) treating a device to present a negatively charged polymer surface layer;
- and
- (b) associating with said polymer surface layer functionalized cationic hyperbranched polymer molecules characterized by having (i) a core moiety of molecular weight 14-1,000 Da (ii) a total molecular weight of 10,000 to 300,000 Da and (iii) a ratio of total molecular weight to core moiety molecular weight of at least 80:1 (e.g. at least 100:1) and bearing one or more negatively charged anti-coagulant entities such as heparin moieties and wherein said functionalized hyperbranched polymer has a net positive charge and is not a dendrimer, wherein the heparin moieties are single point covalently attached to the hyperbranched polymer.
Dependencies are inferred from the claim text.