PARP substrates and biomarkers
AssigneeBoard of Regents, The University of Texas System
InventorYonghao Yu
Life of this patent
Estimating the term…
Abstract
PARylated proteins are enriched by treating cell lysates comprising PARylated proteins and DNA/RNA with an endonuclease that cleaves the DNA/RNA but not the PAR; and separating the PARylated proteins from the cleaved DNA/RNA. PARylation sites are labeled by eluting PARylated proteins from a PAR-affinity substrate with a nucleophilic amine exchange reactant, wherein the reactant labels PARylation sites of the proteins. Specific binding agents are identified by screening compounds for specific binding to a PARylated protein disclosed herein; and identifying one of the compounds as a specific binder of the protein. Antibodies which specifically bind PARylation sites are also disclosed.
Claims20
An antibody which specifically binds derivative of Glu or Asp, wherein the derivative comprises an amide of a nucleophilic amine, the derivative is a reaction product of a nucleophilic amine exchange reagent with an ester of a Glu or Asp residue, the ester is ADP-ribosylated Glu or Asp, the nucleophilic amine exchange reagent is hydroxylamine or hydrazine, and the reaction product is a hydroxamic acid or hydrazide derivative, respectively, of the Asp or Glu residue.
An antibody which specifically binds a derivative of Glu or Asp, wherein the derivative is N-hydroxyl-asparagine, N-hydroxyl-glutamine, N-amine-asparagine or N-amine glutamine.
Dependencies are inferred from the claim text.