Mutants having capability to produce 1, 4-butanediol and method for preparing 1, 4-butanediol using the same
AssigneesLG Chem, Ltd., Korea Advanced Institute of Science and Technology
InventorsSi-Jae Park, Sang-Hyun Lee, Sang-Yup Lee, Eun-Jeong Lee
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Abstract
A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.
Claims20
An isolated mutant microorganism exhibiting high production of 1,4-butanediol, which is prepared by introducing or amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate, and 4-hydroxybutyrate into 1,4-butanediol, in an E. coli capable of producing succinate, wherein the E. coli has inactive genes encoding glucose phosphotransferase (ptsG) and pyruvate kinase (pykA and pykF), and produces succinate in high concentration without substantial production of other organic acids in anaerobic condition,
- wherein the gene encoding the enzyme converting succinate into 4-hydroxybutyrate is selected from the group consisting of genes encoding succinyl-CoA transferase (Cat 1), succinate semialdehyde dehydrogenase (SucD), 4-hydroxybutyrate dehydrogenase (4hbD) and 4-hydroxybutyrate dehydrogenase (GHB).
An isolated mutant microorganism exhibiting high production of 1,4-butanediol, which is prepared by introducing or amplifying a gene encoding Cat1;
- a gene encoding SucD;
- a gene encoding 4hbD or GHB;
- a gene encoding 4-hydroxybutyrate-CoA transferase, or a gene encoding Ptb (phosphotransbutyrylase) and a gene encoding Buk (Butyrylkinase);
- and a gene encoding butyl-CoA dehydrogenase, in E. coli, having inactive genes encoding glucose phosphotransferase (ptsG) and pyruvate kinase (pvkA and pvkF), capable of producing succinate in high concentration in anaerobic condition.
Dependencies are inferred from the claim text.