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US 9,096,843 B2Granted
Peptidyl α-hydroxyglycine α-amidating lyases
AssigneeNovo Nordisk A/S
InventorAllan C. Shaw
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Abstract
The present patent application concerns an enzyme capable of catalysing the conversion of a α-hydroxyglycine to an α-amide and the use of such enzymes for producing a C-terminal α-amidated peptide.
Claims5
A method for producing an α-amidated peptide comprising:
- (i) allowing a target peptide with a C-terminal glycine residue to react with both a peptidylglycine alpha-hydroxylating monooxygenase (PHM) and a polypeptide consisting of amino acids 2-306 of SEQ ID NO:
- 1, amino acids 3-336 of SEQ ID NO:
- 2, amino acids 3-305 of SEQ ID NO:
- 3, or amino acids 3-279 of SEQ ID NO:
- 4, wherein said polypeptide has peptidyl-α-hydroxyglycine alpha-amidating lyase (PAL) activity under conditions suitable for enzymatic activity, and optionally further comprises 1-30 substitution(s) or deletion(s), and wherein the reaction with said PHM and said polypeptide on said target peptide is performed either in two separate steps or simultaneously;
- and
- (ii) recovering the C-terminally α-amidated peptide.
4 more elements
Dependencies are inferred from the claim text.