Detection of toxigenic strains of Clostridium difficile
AssigneeGeneohm Sciences Canada Inc.
InventorsNancy Paquette, Marie-Eve Rochette, Rachel Labourdette
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Abstract
Primers and probes for detection of toxin-producing (toxigenic) strains of Clostridium difficile, and to methods of detecting toxigenic strains using these primers and probes. Toxigenic strains of C. difficile are detected by nucleic acid-based amplification methods using particular primers and probes that bind to the toxin B (TcdB) gene. These primers and probes are used to amplify C. difficile nucleic acids in clinical samples to determine the presence of these toxigenic strains.
Claims15
A method for determining the presence of a toxigenic strain of C. difficile in a biological sample, comprising:
- contacting said sample with at least one pair of primers capable of binding to a C. difficile toxin B (TcdB) gene, wherein each primer in said at least one pair of primers is up to 50 nucleobases in length, and is capable of binding to a C. difficile toxin B (TcdB) gene, wherein said at least one pair of primers comprises a primer comprising the sequence of SEQ ID NO:
- 16 and a primer comprising the sequence of SEQ ID NO:
- 19;
- amplifying target nucleic acid from said sample;
- and
- detecting the presence or amount of an amplified product(s) as an indication of the presence of said toxigenic strain of C. difficile in said sample.
Dependencies are inferred from the claim text.