Chimeric oligomeric compounds and their use in gene modulation
AssigneeIsis Pharmaceuticals, Inc.
InventorStanley T. Crooke
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Abstract
Oligomer compositions comprising first and second oligomers are provided wherein at least a portion of the first oligomer is capable of hybridizing with at least a portion of the second oligomer, at least a portion of the first oligomer is complementary to and capble of hybridizing to a selected target nucleic acid, and at least one of the first or second oligomers includes at least one nucleotide comprising a chimeric organic composition. Oligomer/protein compositions are also provided comprising an oligomer complementary to and capable of hybridizing to a selected target nucleic acid and at least one protein comprising at least a portion of an RNA-induced silencing complex (RISC), wherein at least one nucleotide comprising a chimeric organic composition.
Claims7
A composition comprising a duplex consisting of a first chemically synthesized oligonucleotide and a second chemically synthesized oligonucleotide, wherein:
- each of the first chemically synthesized oligonucleotide and the second chemically synthesized oligonucleotide independently consists of 17 to 25 linked nucleosides, each nucleoside comprising a nucleobase and a sugar;
- the first chemically synthesized oligonucleotide is 100% complementary to the second chemically synthesized oligonucleotide and to a target mRNA;
- the first chemically synthesized oligonucleotide and the second chemically synthesized oligonucleotide are not covalently linked to each other;
- and
- the first chemically synthesized oligonucleotide is a gapmer, wherein the gap comprises at least 4 nucleosides, each comprising a 2′-hydroxy-pentofuranosyl sugar moiety, and wherein each nucleoside of each wing comprises a 2′-sugar modification;
- and
- the second chemically synthesized oligonucleotide comprises at least one nucleoside that comprises a 2′-sugar modification.
Dependencies are inferred from the claim text.