Protein detection
AssigneeAcquascience Limited
InventorsAndre T. R. M. A. Neves, Brian Page
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Abstract
The present invention relates to reagents for separating proteins from detergent, reagents for detecting proteins in the presence of a detergent, and methods of using the same. The separating reagents contain a cyclic oligomer such as cyclodextrin and a cellulose derivative such as 2-hydroxyethyl cellulose. When used in combination with standard protein-complexing dyes, the reagents allow detection of proteins in electrophoresis gels at nanogram levels.
Claims19
A composition for separating a protein from a detergent, said composition comprising a solution, the solution comprising
- a) from 0.05 to 2% w/v of a cellulose derivative,
- b) a cyclic oligomer, and
- c) an α-hydroxycarboxylic acid having a pKa of 4 or less.
A method of detecting and quantifying protein comprising
- i) contacting a protein-containing sample with a first solution comprising a cyclic oligomer and from 0.05 to 2 w/v % of a cellulose derivative;
- ii) contacting the protein-containing sample with a second solution comprising a protein-complexing dye;
- and
- iii) detecting and quantifying the formation of a dye/protein complex.
A method for separating a protein from a detergent, said method comprising
- 1) contacting a sample containing a protein and a detergent with a solution comprising
- a) from 0.05 to 2 w/v % of a cellulose derivative, and
- b) a cyclic oligomer;
- and
- 2) separating the protein from the detergent.
Dependencies are inferred from the claim text.