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US 9,085,778 B2Granted
Exosome transfer of nucleic acids to cells
AssigneeVL27, Inc.
InventorsJan Lötvall, Hadi Valadi
Life of this patent
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Abstract
Methods for introducing nucleic acids to cells via exosomes for use in gene modulation and therapy, such as for gene silencing and to introduce genetic material into cells to compensate for abnormal genes or to induce or repress a process in the recipient cell.
Claims16
A method of transferring genetic material, comprising in this order:
- a) isolating exosomes produced and released by a donor cell;
- b) modifying the isolated exosomes to contain selected nucleic acid constructs;
- and
- c) administering the exosomes containing the selected nucleic acid constructs to recipient cells, wherein administering the exosomes results in the transfer of the specific nucleic acid constructs from the exosomes to the cytoplasm of the recipient cells;
- wherein the isolated exosomes do not contain any substantial amount of nucleic acids;
- and further
- wherein the selected nucleic acid constructs are added to the exosomes that do not contain any substantial amount of nucleic acids.
4 more elements
Dependencies are inferred from the claim text.