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US 9,080,195 B2Granted
High titer production of poly (α 1,3 glucan)
AssigneeE. I. Du Pont de Nemours and Company
InventorsJohn P O'Brien, Mark S Payne
Life of this patent
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Abstract
A process for enzymatic preparation of poly (α1, 3 glucan) from sucrose is disclosed. The glucosyltransferase enzyme (gtfJ) from Streptococcus salivarius is used to convert sucrose to fructose and poly (α1, 3 glucan). Application of semi-permeable membranes to continuously remove fructose, a by-product of the gtf enzyme, thus increasing the poly (α1, 3 glucan) liter, is disclosed.
Claims10
A reaction system for producing poly (alpha 1,3 glucan), wherein said reaction system comprises:
- a) a first chamber that comprises an enzyme reaction solution comprising:
- i) sucrose, and
- ii) at least one glucosyltransferase enzyme that is a Streptococcus salivarius GtfJ enzyme;
- and
- b) a second chamber, separated from the first chamber by a semi-permeable membrane that is in contact with the enzyme reaction solution, wherein the semi-permeable membrane is permeable to fructose and sucrose, but impermeable to poly (alpha 1,3 glucan) and the glucosyltransferase enzyme, and wherein the semi-permeable membrane facilitates continuous removal of fructose and other low molecular weight moieties from the first chamber while retaining poly (alpha 1,3 glucan) and the at least one glucosyltransferase enzyme inside the first chamber, wherein the semi-permeable membrane has a molecular weight cut-off from 12,000 to 100,000 Daltons.
2 more elements
Dependencies are inferred from the claim text.