Method for biocatalytic production of nitriles from oximes and oxime dehydratases usable therein
AssigneeBASF SE
InventorsAndrea Piatesi, Wolfgang Siegel, Kai-Uwe Baldenius
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Abstract
The present invention relates to novel methods for biocatalytic production of nitriles from oximes using oxime dehydratases and novel mutants with oxime dehydratase activity and use thereof in a process for biocatalytic production of nitriles, such as in particular for the production of citral nitrile, neral nitrile, geranial nitrile or citronellyl nitrile from citral oxime, neral oxime, geranial oxime or citronellal oxime; and oxime dehydratases usable therefor, nucleotide sequences therefor and expression constructs or microorganisms comprising these.
Claims17
A biocatalytic process for producing a nitrile of formula I
- RC≡N]n (I)
- wherein
- R is an unsaturated aliphatic hydrocarbon moiety with 8 to 19 carbon atoms;
- and
- n is 1;
- the process comprising
- converting an oxime of general formula II
- RCH═N—OH]n (II)
- in which R and n have the meanings given above, wherein the compound of formula II is in stereoisomerically pure form or as a mixture of stereoisomers,
- to the nitrile of formula I in the presence of a phenylacetaldoxime dehydratase (PAOx) (EC 4.99.1.7) to a compound of formula I, wherein the compound of formula I is in stereoisomerically pure form or as a mixture of stereoisomers.
Dependencies are inferred from the claim text.