Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
AssigneeButamax Advanced Biofuels LLC
InventorBrian James Paul
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Abstract
A high flux of metabolites from pyruvate to 2,3-butanediol in Lactobacillus plantarum was achieved through genetic engineering. Substantial elimination of lactate dehydrogenase activity in the presence of heterologously expressed butanediol dehydrogenase activity led to 2,3 butanediol production that was at least 49% of the total of major pyruvate-derived products.
Claims17
A recombinant lactic acid bacterial cell comprising at least one gene encoding a heterologous polypeptide having butanediol dehydrogenase activity wherein the bacterial cell is substantially free of lactate dehydrogenase activity and wherein the cell produces 2,3-butanediol, and wherein the cell comprises a 2-butanol or 2-butanone biosynthetic pathway.
Dependencies are inferred from the claim text.