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US 9,074,300 B2Granted
Polypeptide immobilization
AssigneeThe University of Chicago
InventorsMilan Mrksich, Christian Hodneland
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Abstract
The present invention provides a method, comprising (a) providing a reactant ligand attached to a substrate; (b) contacting the substrate with a fusion polypeptide, said fusion polypeptide comprising a capture polypeptide fused to a display polypeptide under conditions such that said reactant ligand covalently binds to said capture polypeptide; and (c) analyzing said display polypeptide.
Claims13
A method, comprising:
- a) providing a plurality of hydrolase reactant ligands covalently attached to a substrate;
- b) contacting said substrate and said hydrolase reactant ligands attached to said substrate with a plurality of fusion polypeptides, wherein each of said fusion polypeptides comprising a hydrolase capture polypeptide fused to a display polypeptide, under conditions such that said hydrolase reactant ligands covalently binds to said hydrolase capture polypeptides;
- and
- c) analyzing said display polypeptide;
- wherein said hydrolase reactant ligands reacts specifically with the hydrolase capture polypeptide to form the covalent bond.
2 more elements
Dependencies are inferred from the claim text.