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US 9,074,199 B1Granted
Mutant Cas9 proteins
AssigneePresident and Fellows of Harvard College
InventorsAlejandro Chavez, Frank Poelwijk, George M. Church
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Abstract
Methods of making mutant Cas9 proteins are described.
Claims9
A method of making a mutant Cas9 protein having DNA binding activity comprising
- aligning full length amino acid sequences of a family of Cas9 proteins,
- identifying one or more diverging amino acid sequence portions among the full length amino acid sequences of the family of Cas9 proteins,
- identifying a nucleic acid sequence for the one or more diverging amino acid sequences portions,
- generating a nucleic acid sequence for a target Cas9 protein which lacks the nucleic acid sequence for the one or more diverging amino acid sequences portions, and
- generating a mutant Cas9 protein from the generated nucleic acid sequence.
2 more elements
Dependencies are inferred from the claim text.