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US 9,070,539 B2Granted
Method of charge reduction of electron transfer dissociation product ions
AssigneeMicromass UK Limited
InventorsWeibin Chen, Asish B. Chakraborty, John Charles Gebler, Jeffery Mark Brown
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Abstract
A mass spectrometer is disclosed wherein highly charged fragment ions resulting from Electron Transfer Dissociation fragmentation of parent ions are reduced in charge state within a Proton Transfer Reaction cell by reacting the fragment ions with a neutral superbase reagent gas such as Octahydropyrimidolazepine.
Claims8
A mass spectrometer comprising:
- a cell arranged and adapted to fragment ions by Electron Capture Dissociation and to generate first ions;
- and
- a first device arranged and adapted to react said first ions with one or more neutral, non-ionic or uncharged superbase reagent gases or vapours in order to reduce a charge state of said first ions, wherein said first device comprises a first ion guide comprising a plurality of electrodes, and wherein a majority of said first ions comprise b-type product ions, b-type fragment ions, y-type product ions or y-type fragment ions resulting from the fragmentation of parent or analyte ions by Electron Capture Dissociation.
A method of mass spectrometry comprising:
- fragmenting ions by Electron Capture Dissociation and generating first ions;
- and
- reacting said first ions with one or more neutral, non-ionic or uncharged superbase reagent gases or vapours in order to reduce a charge state of said first ions, wherein a majority of said first ions comprise b-type product ions, b-type fragment ions, y-type product ions or y-type fragment ions resulting from the fragmentation of parent or analyte ions by Electron Capture Dissociation.
Dependencies are inferred from the claim text.