Reagent system and method
AssigneeLeica Biosystems Melbourne Pty Ltd
InventorCosta Conn
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Abstract
The present invention relates generally to a chromogenic compositions and reagents systems and methods therefor.
Claims19
A chromogenic reagent system comprising a first solution comprising a nitrite, a second solution comprising an aryl amine selected from the group comprising 3-amino-4-methoxybenzamide, 2-amino-4-methoxybenzamide, 4-aminobenzoic acid, 3-amino-4-methoxybenzoic acid, 2-aminoterephthalic acid, 2-amino-5-hydroxybenzoic acid, 2-amino-4-chlorobenzoic acid, 2-amino-4,5-dimethoxybenzoic acid aniline, 4-nitroanline, 2-methoxyanline, 4-chloro-2-methylaniline and mixtures thereof, a third solution comprising an acid and a fourth solution comprising a naphthol phosphate.
A method of preparing a chromogen composition, the method comprising:
- combining a first solution comprising a nitrite and a second solution comprising an aryl amine selected from the group comprising 3-amino-4-methoxybenzamide, 2-amino-4-methoxybenzamide, 4-aminobenzoic acid, 3-amino-4-methoxybenzoic acid, 2-aminoterephthalic acid, 2-amino-5-hydroxybenzoic acid, 2-amino-4-chlorobenzoic acid, 2-amino-4,5-dimethoxybenzoic acid, aniline, 4-nitroanline, 2-methoxyanline, 4-chloro-2-methylaniline and mixtures thereof;
- to which is added a third solution comprising an acid to produce a diazonium compound;
- and
- combining said diazonium compound with a fourth solution comprising a naphthol phosphate.
A reagent system comprising a first solution comprising a nitrite, a second solution comprising 3-amino-4-methoxybenzamide in an acidic solution and a third solution comprising a naphthol phosphate.
A method of staining a biological specimen, comprising:
- (i) combining a first solution comprising a nitrite and a second solution comprising an aryl amine selected from the group comprising 3-amino-4-methoxybenzamide, 2-amino-4-methoxybenzamide, 4-aminobenzoic acid, 3-amino-4-methoxybenzoic acid, 2-aminoterephthalic acid, 2-amino-5-hydroxybenzoic acid, 2-amino-4-chlorobenzoic acid, 2-amino-4,5-dimethoxybenzoic acid, aniline, 4-nitroanline, 2-methoxyanline, 4-chloro-2-methylaniline and mixtures thereof;
- to which is added a third solution comprising an acid to produce a diazonium compound;
- combining said diazonium compound with a fourth solution comprising a naphthol phosphate to produce a chromogen composition;
- and
- (ii) applying said chromogen composition to said biological specimen.
A chromogenic reagent system comprising a first solution comprising a nitrite, a second solution comprising 3-amino-4-methoxybenzamide in an acidic solution, wherein the molar ratio of acid to 3-amino-4-methoxybenzamide is about 1:1 or less;
- a third solution comprising an acid and a fourth solution comprising a naphthol phosphate.
Dependencies are inferred from the claim text.