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US 9,068,981 B2Granted
Lateral flow assays with time delayed components
AssigneeRapid Pathogen Screening, Inc.
InventorsUma Mahesh Babu, Robert W. VanDine, Robert P. Sambursky
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Abstract
A lateral flow device may include one or more enhancement elements, where the enhancement elements bind to the analyte sandwich to increase a detection signal in the test zone. In preferred embodiments, some or all of the enhancement elements are encapsulated.
Claims21
A lateral flow device for detecting an analyte comprising:
- a test strip comprising a sample application zone and a test zone;
- a first conjugate comprising a first binding partner for the analyte and a label;
- a second binding partner for the analyte capable of being immobilized in the test zone;
- and
- at least one encapsulated enhancement element;
- wherein the analyte, the conjugate, and the second binding partner form a sandwich which is immobilized in the test zone when the analyte is present;
- and
- wherein the encapsulated enhancement element binds to the sandwich after being released from encapsulation to increase a detection signal in the test zone.
5 more elements
A method of enhancing a signal on a lateral flow device comprising a test strip comprising a sample application zone and a test zone, a conjugate comprising a first binding partner for an analyte in a sample and a label, a second binding partner for the analyte;
- and an encapsulated enhancement element, comprising the steps of:
- a) immobilizing a sandwich formed between the analyte, if present in the sample, the conjugate, and the second binding partner in a test zone of the lateral flow device;
- b) delaying release of the enhancement element due to encapsulation;
- and
- c) binding the enhancement element to the sandwich after being released from encapsulation such that the enhancement element increases a detection signal in the test zone.
2 more elements
Dependencies are inferred from the claim text.