Micro bio sensor and method for manufacturing the micro bio sensor
AssigneeSeiko Epson Corporation
InventorsHitoshi Fukushima, Akira Nishimura, Mitsuhiro Ban, Asahi Yamazaki, Takuya Asai
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Abstract
A micro bio sensor which detects a microbe existing in a specimen is provided. The microbial sensor includes a base, a detector formed on the base, and a reaction layer formed on the detector, wherein the reaction layer is comprised of a self-assembled monolayer which is formed on the detector and an antibiotic which is immobilized through the self-assembled monolayer on the detector. By using the micro bio sensor, it is possible to detect species of the microbe concurrently and improve sensitivity for detecting the species of the microbe. Further, a method for manufacturing such a micro bio sensor is also provided.
Claims10
A micro bio sensor which detects a microbe existing in a specimen, the micro bio sensor comprising:
- a detector disposed on a base, the detector including a first electrode;
- and
- a reaction layer disposed on the detector, and the microbe being detected by the detector due to bonding between the reaction layer and the microbe,
- the reaction layer including:
- a self-assembled monolayer which includes a plurality of molecules, each of the plurality of molecules having one end and an other end, the one end bonding with the first electrode;
- a first biotin which is disposed at the other end of at least one of the plurality of molecules;
- streptavidin which is bonded with the first biotin;
- and
- a complex functional molecule which includes a second biotin, a polyethylene glycol chain and a penicillin antibiotic, an amide bonding being located between the second biotin and the polyethylene glycol chain and between the penicillin antibiotic and the polyethylene glycol chain, the second biotin being bonded with the streptavidin.
A method for manufacturing a micro bio sensor, the method comprising:
- forming a detector on a base;
- and
- forming a reaction layer on the detector, and a microbe being detected by the detector due to bonding between the reaction layer and the microbe;
- the forming of the reaction layer on the detector including:
- preparing a complex functional molecule which includes a second biotin, a polyethylene glycol chain and a penicillin antibiotic, an amide bonding being located between the second biotin and the polyethylene glycol chain and between the penicillin antibiotic and the polyethylene glycol chain;
- forming a self-assembled monolayer which includes a plurality of molecules, each of the plurality of molecules having one end and an other end, a first biotin being disposed at the other end of at least one of the plurality of molecules;
- performing steptavidin treatment to bond a steptavidin to the first biotin;
- and
- immobilizing the complex functional molecules to bond the second biotin to the streptavidin.
A micro bio sensor which detects a microbe existing in a specimen, the micro bio sensor comprising:
- a detector disposed on a base, the detector including a first electrode;
- and
- a reaction layer disposed on the detector, and the microbe being detected by the detector due to bonding between the reaction layer and the microbe,
- the reaction layer including:
- a self-assembled monolayer which includes a plurality of molecules, each of the plurality of molecules having one end and the other end, the one end bonding with the first electrode;
- a first biotin which is disposed at the other end of at least one of the plurality of molecules;
- streptavidin which is bonded with the first biotin;
- and
- a complex functional molecule which includes a second biotin, a polyethylene glycol chain and an antibiotic;
- wherein the second biotin is bonded to the antibiotic through the polyethylene glycol chain, and the second biotin is bonded with the streptavidin.
Dependencies are inferred from the claim text.