US 9,068,976 B2Granted

Integrated filtration bioanalyzer

AssigneePhotonic BioSystems, Inc.

InventorsDavid L. Putnam, Jason A. Putnam, Todd W. Hubbard, James A. van Zee

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Abstract

The present invention relates to an in vitro assay method and device that provides for detection and measurement of entities in a fluid sample that can be captured and concentrated in a unitized self-contained enclosed filter apparatus that is analyzed in an optical detection instrument for indications of the entity. It provides for analysis of biological material including cells, their enzymes, or other constituents thereof, that can be identified based on an indicator-generating means. The analysis provided for include detection of the presence of the entity, and changes in the entity over time, such as associated with growth and increasing metabolic activity with an expanding population of cells, or decreasing metabolic activity, for example, due to presence of inhibitory or toxic agents.

Claims33

Dependent claims

An in vitro method for determining the presence and/or activity of biological material in a liquid sample, comprising the steps of:

(i) filtering a liquid sample through an enclosed filter apparatus capable of trapping said biological material in said filter apparatus, whereby said biological material present in said liquid sample is retained in said filter apparatus;
(ii) administering a reaction fluid into and/or through said filter apparatus, said reaction fluid comprising:
i. components to support and/or affect the biological activity of said biological material and/or,
ii. an indicator means for indicating the presence and/or activity of said biological material;
(iii) incubating said filter apparatus, said biological material retained and said reaction fluid in a measurement apparatus;
(iv) performing one or more measurements by optical means of said indicator means and/or said biological material concurrent with incubation of said biological material in said filter apparatus using said measurement apparatus;
and
(v) performing analysis of said measurements of the said indictor means and/or said biological material to determine activity of the said biological material,
wherein steps (i)-(v) are performed within the enclosed filter apparatus.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application: 12/582,392Filed 20 Oct 2009 · us provisional application
Priority: 20 Oct 2008
Published: US 2010/0129852 A127 May 2010
Granted: 30 Jun 2015
Term ends: Not estimatedThe source records don’t support an estimate.

Classification5 codes

G01N 33/52
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing: use of…
C12Q 1/24
Involving viable microorganisms: methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
G01N 2001/4088
By other techniques involving separation of suspended solids: filtration
G01N
Investigating or analysing materials by determining their chemical or physical properties