Optical analysis device, optical analysis method and computer program for optical analysis using single light-emitting particle detection

An optical analysis method of detecting light from a light-emitting particle dispersed and moving at random in a sample solution using an optical system of a confocal microscope or a multiphoton microscope, comprising:

• (a) moving a position of a light detection region of the optical system in the sample solution;
• (b) detecting light from the light detection region during moving the position of the light detection region in the sample solution and generating time series light intensity data;
• and
• (c) detecting a signal indicating light from each light-emitting particle individually on the time series light intensity data;
• wherein the method comprises (d) removing regions where no signal indicating light from each light-emitting particle exists in the time series light intensity data from the time series light intensity data, and
• wherein the method further comprises smoothing data values corresponding to light intensity variation over a plurality of units of time of a light signal from the particle.

A non-transitory computer readable storage device having a computer program product including programmed instructions for optical analysis of detecting light from a light-emitting particle dispersed and moving at random in a sample solution using an optical system of a confocal microscope or a multiphoton microscope, said programmed instructions causing a computer to perform steps of:

• moving a position of a light detection region of the optical system in the sample solution;
• detecting light from the light detection region during moving the position of the light detection region in the sample solution generating time series light intensity data;
• and
• detecting a signal from each light-emitting particle individually in the time series light intensity data,
• wherein said programmed instructions further causes a computer to perform a step of removing regions where no signal indicating light from each light-emitting particle exists in the time series light intensity data from the time series light intensity data
• wherein said programmed instructions further causes the computer to smooth data values corresponding to light intensity variation over a plurality of units of time of a light signal from the particle.