Method of analysis of genetic markers
AssigneeLife Technologies Corporation
InventorsBarry Merriman, Paul Mola
Life of this patent
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Abstract
A method of analyzing genetic markers includes binding a set of probes to a segment of single stranded nucleic acids. The segment of single stranded nucleic acids includes a repeat region formed of at least two of a repeat unit. The repeat unit can include at least two nucleic acids. The set of probes includes a first probe complementary to the repeat unit. The method can further include directing the segment through a nanopore device and measuring a signal through the nanopore device. The signal can be indicative of the number of repeat units.
Claims10
A method of detecting an allele of a human identification panel, the method comprising:
- binding specifically a set of probes to a single stranded nucleic acid segment derived from a locus of the human identification panel, the segment including a microsatellite region having between 5 and 30 k-mer repeat units, wherein k is in a range of 2 to 6, the set of probes including a first subset of k-mer binding probes complementary to the k-mer repeat units;
- directing the segment through a porin protein nanopore of a nanopore device with an applied voltage, the bound set of probes being stripped from the segment as the segment passes through the porin protein nanopore;
- measuring with the nanopore device an ion current signal including current spikes corresponding to the stripping of probes of the first subset of k-mer binding probes as the segment passes through the protein nanopore of the nanopore device, a number of current spikes of the ion current signal indicative of the number of k-mer repeat units;
- detecting the allele based on a characteristic of the current spikes of the ion current signal, wherein the characteristic includes the number of the current spikes.
Dependencies are inferred from the claim text.