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AssigneeSolazyme, Inc.
InventorsScott Franklin, Aravind Somanchi, George Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
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Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
A recombinant oleaginous cell of the genus Prototheca, the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca, wherein the cell produces an oil elevated in erucic acid due to the expression of the exogenous gene.
A recombinant oleaginous cell of the genus Prototheca, the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca, wherein the cell produces an oil elevated in eicosenoic acid due to the expression of the exogenous gene.
A recombinant oleaginous cell of the genus Prototheca, the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca, wherein the cell produces an oil elevated in docosadienoic acid due to the expression of the exogenous gene.
A recombinant oleaginous cell of the genus Prototheca, the cell comprising an exogenous gene encoding an active ketoacyl-CoA synthase enzyme of Cramble abyssinica, Lunaria annua or Cardamine graeca, wherein the cell produces an oil elevated in C22:5 due to the expression of the exogenous gene.
Dependencies are inferred from the claim text.