Back to results
US 9,068,212 B2Granted
Method for producing a polypeptide using a cell that overexpresses a bicarbonate transporter
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsHisahiro Tabuchi, Satoshi Tainaka, Tomoya Sugiyama
Life of this patent
Estimating the term…
Abstract
The present invention provides a method capable of producing a protein efficiently. A method of producing a polypeptide, comprising culturing a cell which strongly expresses a bicarbonate transporter and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce said polypeptide.
Claims7
A method of producing an antibody comprising
- culturing an isolated mammalian cell which is transfected with a heterologous DNA encoding a bicarbonate transporter and is transfected with a heterologous DNA encoding a desired antibody and allowing the cell to produce said antibody,
- wherein the bicarbonate transporter exchanges Cl− outside of a plasma membrane for HCO3− inside a plasma membrane, and wherein the bicarbonate transporter is anion exchanger 1 (AE1), anion exchanger 2 (AE2), or anion exchanger 3 (AE3).
An isolated mammalian cell which is transfected with a heterologous DNA encoding a bicarbonate transporter and is transfected with a heterologous DNA encoding a desired antibody,
- wherein the bicarbonate transporter exchanges Cl− outside of a plasma membrane for HCO3− inside a plasma membrane, and wherein the bicarbonate transporter is anion exchanger 1 (AE1), anion exchanger 2 (AE2), or anion exchanger 3 (AE3).
Dependencies are inferred from the claim text.