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US 9,068,201 B2Granted
Methods and compositions for producing fatty alcohols
AssigneeReg Life Sciences, LLC
InventorsZhihao Hu, Vikranth Arlagadda
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Abstract
Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described.
Claims18
A bacterial cell culture comprising:
- (a) a bacterial cell genetically engineered to express an exogenous polypeptide having the amino acid sequence of SEQ ID NO:22, and
- (b) a glycerol-containing carbon source,
- wherein a fatty alcohol composition comprising C12 and C14 fatty alcohols is produced by the genetically engineered bacterial cell.
A bacterial cell genetically engineered to express
- (a) an exogenous polypeptide having the amino acid sequence of SEQ ID NO:22,
- (b) a polypeptide having thioesterase (EC 3.1.2.14 or EC 3.1.1.5) activity, and
- (c) a polypeptide having attenuated acyl-CoA dehydrogenase (fadE;
- EC 1.3.99.3, EC 1.3.99.) activity,
- wherein the genetically engineered bacterial cell produces a fatty alcohol composition comprising C12 and C14 fatty alcohols when cultured in the presence of a glycerol-containing carbon source.
2 more elements
A method of making a fatty alcohol composition, comprising:
- a. culturing a bacterial cell genetically engineered to express an exogenous polypeptide having the amino acid sequence of SEQ ID NO:22 in a culture medium comprising glycerol;
- b. fermenting the genetically engineered bacterial cell of part a in a fermentation media, wherein a fatty alcohol composition comprising C12 and C14 fatty alcohols is produced by the genetically engineered bacterial cell;
- and
- c. optionally separating the fatty alcohol composition from the fermentation media.
1 more element
Dependencies are inferred from the claim text.