Methods for enhancing stress tolerance in plants and compositions thereof
AssigneeMonsanto Technology LLC
InventorMary Fernandes
Life of this patent
Estimating the term…
Abstract
Increased tolerance to abiotic stress in a plant is provided by introducing DNA expressing a cold shock protein, e.g. bacterial cold shock protein.
Claims29
A drought tolerant transgenic plant that comprises in its genome a recombinant DNA molecule that expresses a cold shock protein, wherein said cold shock protein comprises the cold shock domain sequence [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] of SEQ ID NO:3, has at least 60% identity across the entire length of the Escherichia coli CspA protein of SEQ ID NO:1, and confers resistance to drought.
A method of improving drought tolerance in a transgenic progeny plant comprising crossing a plant with a drought tolerant transgenic plant having a recombinant DNA expressing a cold shock protein wherein said cold shock protein comprises a cold shock domain sequence [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] of SEQ ID NO:3, has at least 60% identity across the entire length of the Escherichia coli CspA protein of SEQ ID NO:1, and confers resistance to drought, and obtaining a transgenic progeny plant having said recombinant DNA.
A method of producing a drought tolerant transgenic plant comprising the steps of:
- a) inserting into the genome of plant cells a recombinant DNA molecule that comprises, in the 5′ to 3′ direction:
- (i) a first DNA polynucleotide comprising a promoter that functions in plants and which is operably linked to
- (ii) a second DNA polynucleotide that encodes a cold shock protein that comprises a cold shock domain sequence [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] of SEQ ID NO:3, has at least 60% identity across the entire length of Escherichia coli CspA protein of SEQ ID NO:1, and which is operably linked to
- (iii) a 3′ transcription termination DNA polynucleotide that functions as a polyadenylation sequence;
- b) obtaining transformed plant cells containing said recombinant DNA;
- c) regenerating transgenic plants from said plant cells;
- and
- d) selecting a transgenic plant having increased drought tolerance.
A method for increasing yield in a crop subject to water deficit during its growth, said method comprising planting seeds having a recombinant DNA expressing a cold shock protein, wherein said cold shock protein comprises the cold shock domain sequence [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] of SEQ ID NO:3, has at least 60% identity across the entire length of the Escherichia coli CspA protein of SEQ ID NO:1 and confers resistance to drought, and allowing said seeds to grow to mature plants under drought conditions.
Dependencies are inferred from the claim text.