Treatment of uncoupling protein 2 (UCP2) related diseases by inhibition of natural antisense transcript to UCP2
AssigneeCuRNA, Inc.
InventorsJoseph Collard, Olga Khorkova Sherman
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Abstract
The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Uncoupling Protein 2 (UCP2), in particular, by targeting natural antisense polynucleotides of Uncoupling Protein 2 (UCP2). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of UCP2.
Claims12
A method of upregulating a function of and/or the expression of a Uncoupling Protein 2 (UCP2) polynucleotide in patient cells or tissues in vivo or in vitro, said patient having a UCP2 associated disease or disorder, comprising:
- contacting said cells or tissues with at least one antisense oligonucleotide of 12-30 nucleotides in length that specifically targets and specifically hybridizes to a region of a natural antisense oligonucleotide of the Uncoupling Protein 2 (UCP2) polynucleotide selected from SEQ ID NOS:
- 2 or 3;
- thereby upregulating a function of and/or the expression of the Uncoupling Protein 2 (UCP2) polynucleotide in patient cells or tissues in vivo or in vitro.
A method of upregulating a function of and/or the expression of a Uncoupling Protein 2 (UCP2) gene in mammalian cells or tissues in vivo or in vitro comprising:
- contacting said cells or tissues with at least one short interfering RNA (siRNA) oligonucleotide 19 to 30 nucleotides in length, wherein said at least one siRNA oligonucleotide specifically hybridizes to a region of a natural antisense polynucleotide of a Uncoupling Protein 2 (UCP2) polynucleotide selected front SEQ ID NOS:
- 2 or 3, and upregulates a function of and/or the expression of Uncoupling Protein 2 (UCP2) in mammalian cells or tissues in vivo or in vitro.
A method of upregulating a function of and/or the expression of Uncoupling Protein 2 (UCP2) in mammalian cells or tissues in vivo or in vitro comprising:
- contacting said cells or tissues with at least one single stranded modified antisense oligonucleotide of about 20 to 30 nucleotides in length that specifically hybridizes to a natural antisense strand of a Uncoupling Protein 2 (UCP2) polynucleotide selected from SEQ ID NOS:
- 2 or 3 wherein said at least one antisense oligonucleotide has at least 90% sequence identity to at least one nucleic acid sequence of about 20 to 30 nucleotides set forth within SEQ ID NO:
- 1 or a polynucleotide encoded by the UCP2 gene;
- and, upregulating the function and/or expression of the Uncoupling Protein 2 (UCP2) in mammalian cells or tissues in vivo or in vitro.
Dependencies are inferred from the claim text.