Polypeptides having endoglucanase activity and polynucleotides encoding same

A nucleic acid construct comprising a polynucleotide encoding a polypeptide having a catalytic domain, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide in an expression host, and wherein the catalytic domain is selected from:

• (a) a catalytic domain having at least 95% sequence identity to amino acids 19 to 397 of SEQ ID NO:
• 2;
• (b) a catalytic domain encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) nucleotides 55 to 1191 of SEQ ID NO:
• 1, or (ii) the full-length complement of (i), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.;
• and
• (c) a catalytic domain encoded by a polynucleotide having at least 95% sequence identity nucleotides 55 to 1191 of SEQ ID NO:
• 1.

A nucleic acid construct comprising a polynucleotide encoding a polypeptide having a cellulose binding domain linked to a catalytic domain, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct production of the polypeptide in an expression host, and wherein the binding domain is selected from:

• (a) a cellulose binding domain having at least 95% sequence identity to amino acids 444 to 477 of SEQ ID NO:
• 2;
• (b) a cellulose binding domain encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) nucleotides 1333 to 1431 of SEQ ID NO:
• 1, or (ii) the full-length complement of (i), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15minutes using 2×SSC, 0.2% SDS at 70° C.;
• and
• (c) a cellulose binding domain encoded by a polynucleotide having at least 95% sequence identity to nucleotides 1333 to 1431 of SEQ ID NO:
• 1.

A method for degrading or converting a cellulosic material, said method comprising:

• (i) treating the cellulosic material with an enzyme composition in the presence of a polypeptide having endoglucanase activity;
• and
• (ii) recovering the degraded cellulosic material;
• wherein the polypeptide having endoglucanase activity is selected from:
• (a) a polypeptide having at least 95% sequence identity to amino acids 19 to 477 of SEQ ID NO:
• 2;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) nucleotides 55 to 1434 of SEQ ID NO:
• 1, or (ii) the full-length complement of (i), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.;
• and
• (c) a polypeptide encoded by a polynucleotide having at least 95% sequence identity to nucleotides 55 to 1434 of SEQ ID NO:
• 1.

A method for producing a fermentation product, said method comprising:

• (i) saccharifying a cellulosic material with an enzyme composition in the presence of a polypeptide having endoglucanase activity;
• (ii) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product;
• and
• (iii) recovering the fermentation product from the fermentation;
• wherein the polypeptide having endoglucanase activity is selected from:
• (a) a polypeptide having at least 95% sequence identity to amino acids 19 to 477 of SEQ ID NO:
• 2;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) nucleotides 55 to 1434 of SEQ ID NO:
• 1, or (ii) the full-length complement of (i), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.;
• and
• (c) a polypeptide encoded by a polynucleotide having at least 95% sequence identity to nucleotides 55 to 1434 of SEQ ID NO:
• 1.

A method of fermenting a cellulosic material, said method comprising:

• (i) fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition in the presence of a polypeptide having endoglucanase activity, and wherein the fermenting of the cellulosic material produces a fermentation product;
• and
• (ii) recovering the fermentation product from the fermentation;
• wherein the polypeptide having endoglucanase activity is selected from:
• (a) a polypeptide having at least 95% sequence identity to amino acids 19 to 477 of SEQ ID NO:
• 2;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) nucleotides 55 to 1434 of SEQ ID NO:
• 1, or (ii) the full-length complement of (i), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.;
• and
• (c) a polypeptide encoded by a polynucleotide having at least 95% sequence identity nucleotides 55 to 1434 of SEQ ID NO:
• 1.