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US 9,068,170 B2Granted
Generation of pluripotent stem cells using recombinant proteins
AssigneeThe Scripps Research Institute
InventorsHongyan Zhou, Sheng Ding
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Abstract
The present invention provides for methods, compositions, and kits of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell using exogenous transcription factors.
Claims14
A method of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell, the method comprising:
- contacting the non-pluripotent cell with a plurality of exogenous transcription factor polypeptides, each exogenous transcription factor polypeptide comprising a transcription factor fused to a heterologous peptide sequence that enhances transport across cell membranes, under conditions to produce the induced pluripotent stem cell from the mammalian non-pluripotent cell;
- wherein the exogenous transcription factor polypeptides are selected from the group consisting of:
- (i) Oct4, Klf4, and Sox2;
- and (ii) Oct4, Klf4, Sox2, and c-Myc;
- and
- contacting the non-pluripotent cell with a MEK inhibitor, a GSK3 inhibitor, and a TGFβ receptor inhibitor/ALK5 inhibitor.
3 more elements
Dependencies are inferred from the claim text.