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US 9,068,169 B2Granted
CD56 positive human adult pancreatic endocrine progenitor cells
AssigneeReneuron Limited
InventorsWen-Ghih Tsang, Tianli Zheng, Wei Liu
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Abstract
The invention relates to the discovery of a selective cell surface marker that permits the selection of a unique subset of pancreatic stems cells having a high propensity to differentiate into insulin producing cells or into insulin producing cell aggregates.
Claims14
A method of obtaining a culture of propagating pancreatic cells that exhibit a CD56 protein as a cell surface marker comprising:
- (a) isolating a population of cells from a pancreas that include undifferentiated pancreatic cells and differentiated pancreatic cells;
- (b) contacting the population of cells with a CD56 binding reagent;
- (c) selecting the undifferentiated pancreatic cells that specifically bind to the CD56 binding reagent, wherein the undifferentiated pancreatic cells can be expanded in culture;
- and (d) separating the selected, undifferentiated pancreatic cells from the differentiated pancreatic cells that do not bind the CD56 binding reagent to obtain a culture of undifferentiated propagating pancreatic cells that exhibit the CD56 protein as a cell surface marker.
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