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US 9,068,163 B2Granted
Methods and compositions for growth of embryonic stem cells
AssigneeThe Regents of the University of Michigan
InventorsGary D. Smith, Joerg Lahann, Jun Ding, Himabindu Nandivada
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Abstract
The present invention provides methods and compositions for establishing and maintaining growth of undifferentiated stem cells. In particular, the present invention provides synthetic growth matrices for stem cells, wherein said cells are capable of going through multiple passages while remaining in an undifferentiated state.
Claims3
A method for culturing human pluripotent stem cells comprising:
- a) applying human pluripotent stem cells to a substrate, wherein said substrate comprises poly[2-(methacryloyloxy)ethyl dimethyl(3-sulfopropyl)ammonium] (PMEDSAH);
- and
- b) growing said human stem cells on said substrate in the presence of culture media capable of maintaining said cells.
Dependencies are inferred from the claim text.